Defective natural killer cytotoxicity and polymorphonuclear leukocyte antibody-dependent cellular cytotoxicity in patients with LFA-1/OKM-1 deficiency.
نویسندگان
چکیده
Four children with an immunodeficiency involving the absence of leukocyte membrane glycoproteins reacting with anti-LFA-1 and OKM-1 monoclonal antibodies were unable to mediate adherence-dependent leukocyte functions. Even with normal Fc receptor function, their PMN-ADCC and MC-NKC were markedly deficient. Single cell analysis demonstrated deficient antibody-mediated PMN-target cell adherence. Monoclonal antibodies against LFA-1 and OKM-1 reproduced this immunodeficiency in leukocytes from normal adults. LFA-1/OKM-1 mediates a PMN-target cell adhesive step.
منابع مشابه
Enhancement of the antibody-dependent cellular cytotoxicity of low-fucose IgG1 Is independent of FcgammaRIIIa functional polymorphism.
PURPOSE The most common polymorphic variant of Fcgamma receptor type IIIa (FcgammaRIIIa), FcgammaRIIIa-158F, has been associated with inferior clinical responses to anti-CD20 chimeric IgG1 rituximab compared with FcgammaRIIIa-158V. As we previously found that removal of fucose residues from the oligosaccharides of human IgG1 results in enhanced antibody-dependent cellular cytotoxicity, we compa...
متن کاملEnhancement of the Antibody-Dependent Cellular Cytotoxicity of Low-Fucose IgG1 Is Independent of Fc RIIIa Functional Polymorphism
Purpose: The most common polymorphic variant of Fc receptor type IIIa (Fc RIIIa), Fc RIIIa-158F, has been associated with inferior clinical responses to anti-CD20 chimeric IgG1 rituximab compared with Fc RIIIa-158V. As we previously found that removal of fucose residues from the oligosaccharides of human IgG1 results in enhanced antibody-dependent cellular cytotoxicity, we compared the effects ...
متن کاملNatural and Cytotoxicity Antibody-Dependent Cellular of Polymorphonuclear Leukocytes
OHARA, M., SHIRADO, M., MIYATA, M., SAKUMA, H., NANAMIYA, M., YOSHIDA, N., YOSHIDA, H. and KASUKAWA, R. Natural and Antibody-Dependent Cellular Cytotoxicity o f Polymorphonuclear Luekocytes. Tohoku J. exp. Med., 1983, 140 (1), 59-66 Cytotoxic activity of polymorphonuclear leukocytes (PMN) in the peripheral blood of patients with various diseases was demonstrated to K562 cells (natural cytotoxic...
متن کاملAntibody-dependent cytotoxicity mediated by natural killer cells is enhanced by castanospermine-induced alterations of IgG glycosylation.
Inhibitors of glycosylation and carbohydrate processing were used to probe the functional consequences of specific, differential alterations in glycosylation of monoclonal IgG secreted by hybridoma clones. Neither the absence of glycosylation nor the presence of atypical oligosaccharides significantly influenced binding of the monoclonal antibody to the cell surface antigen recognized. However,...
متن کاملBy Francisco Sanchez - Madrid , Janice a . Nagy , Elizabeth Robbins , Paul Simon , and Timothy
Two human molecules with surprisingly similar molecular structures, LFA-1 and Mac-1/OKM1, have recently been found to be important in cytolytic T lymphocyte-mediated killing, and in complement receptor function, respectively. Human LFA-1 contains two subunits of Mr 177,000 and 95,000 (1), and OKM 1/ Mac-1 (2) has two polypeptides of strikingly similar size. Monoclonal antibodies (MAb) to human ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of immunology
دوره 133 6 شماره
صفحات -
تاریخ انتشار 1984